What do you think would happen if you reduce the incubation time to 30 minutes for tube 5?

What do you think would happen if you reduce the incubation time to 30 minutes for tube 5?

What do you think would happen if you reduce the incubation time to 30 minutes for tube 5 (pepsin, BAPNA, pH 7.0 buffer)? With more enzyme activity the optical density increases. If I reduced the incubation time to 30 minutes for tube 5, optical density would likely decrease or be 0.00.

What is the significance of using 37 degrees Celsius for incubator?

The air in the incubator was kept at 37 degrees Celsius, the same temperature as the human body, and the incubator maintained the atmospheric carbon dioxide and nitrogen levels necessary to promote cell growth. At this time, incubators also began to be used in genetic engineering.

What happens to the lipid digestion when you change the incubation temperature?

Increasing temperatures from 0 ºC to around 45 ºC will reduce the time taken for the lipase to break down the fat in milk. Over this temperature, the time taken will increase, or perhaps the lipase will not work at all.

What effect did freezing have on enzyme activity?

Freezing had no effect on enzyme activity. Boiling negatively effected the enzymes activity. Temperature effects the ability of an enzyme to react, At what pH was the amylase most active?

What was the effect of freezing on the activity of salivary amylase?

Freezing does not change the structure of the enzyme so it doesn’t have an effect on the activity.

What was pepsin effect on bapna?

Protein Digestion by Pepsin: What effect did boiling have on pepsin? Boiling pepsin denatured the enzyme causing it to have no effect on BAPNA.

What effect did Boiling have on pepsin?

Why do we incubate bacteria at 25 degrees?

Inoculated agar plates are incubated at 25°C in school laboratories for no more than 24–48 hours. This encourages growth of the culture without growing human pathogens which thrive at body temperature (37°C).

How does temperature affect pepsin?

Changing temperature usually radically affects the catalytic performance of enzymes. When testing the effect of temperature on pepsin enzyme activity, the results showed that pepsin worked best at the temperature 30 °C. When the temperature decreased to 22 °C, the enzyme activity decreased sharply.

What is the effect of pepsin?

Of these five components, pepsin is the principal enzyme involved in protein digestion. It breaks down proteins into smaller peptides and amino acids that can be easily absorbed in the small intestine.

How do low temperatures affect enzymes?

Temperature. At low temperatures, the number of successful collisions between the enzyme and substrate is reduced because their molecular movement decreases. The reaction is slow. The human body is maintained at 37°C as this is the temperature at which the enzymes in our body work best.

How does temperature affect enzyme activities?

As with many chemical reactions, the rate of an enzyme-catalysed reaction increases as the temperature increases. An optimum activity is reached at the enzyme’s optimum temperature. A continued increase in temperature results in a sharp decrease in activity as the enzyme’s active site changes shape.

How does the pH of pepsin affect its stability?

In a second experiment we determined the effects of different concentrations of glycerol on the preservation of peptic activity. From these experiments we conclude that pepsin is unstable when stored frozen at low pH but not when stored above pH 2.

Which is the best temperature for pepsin to be active?

The same applies to the temperatures above 40°c and indicates 40°c is the best temperature range for pepsin to be active.

What does a lower reading on pepsin mean?

Therefore the lower the reading means less light has been absorbed by the solution being tested indicating in this case that more protein (egg albumen) has been digested by the enzyme (pepsin) and the lower the reading the faster the enzyme reaction rate.

What is the substrate of the subunits of pepsin?

The substrate of pepsin is BAPNA and the usual substrate is protein. The subunits are amino acids and peptides What was the effect of dicreaseing the incubation time on the optical density results?