What species can grow on mannitol salt agar?

What species can grow on mannitol salt agar?

The MSA will select for organisms such as Staphylococcus species which can live in areas of high salt concentration (plate on the left in the picture below). This is in contrast to Streptococcus species, whose growth is selected against by this high salt agar (plate on the right in the picture below).

What bacteria does mannitol salt agar inhibit?

It contains a high concentration (about 7.5–10%) of salt (NaCl) which is inhibitory to most bacteria – making MSA selective against most Gram-negative and selective for some Gram-positive bacteria (Staphylococcus, Enterococcus and Micrococcaceae) that tolerate high salt concentrations.

Can gram-negative bacteria grow on mannitol salt agar?

Gram-negative bacteria like E. coli and P. aeriginosa are not tolerant to salt (not halophilic) and will not grow colonies on MSA (see quadrants II and IV). Pathogenic gram-positive Staphylococcus aureus salt-tolerant (halophilic) and will produce colonies on MSA.

Does S aureus grow on MSA?

Mannitol Salt Agar is differential due to the presence of mannitol and the pH indicator Phenol Red. aureus grow on Mannitol Salt Agar. About 8 % to 12 % of Staph. aureus strains will not ferment mannitol.

Can Streptococcus grow on mannitol salt agar?

Both streptococcal organisms are catalase negative and beta-hemolytic on sheep blood agar plates. Also, neither would grow on the mannitol salt agar. Streptococcus pyogenes is sensitive to growth inhibition by bacitracin, whereas Streptococcus agalactiae (group B streptococci) is not.

What bacteria can ferment mannitol?

Most pathogenic staphylococci, such as Staphylococcus aureus, will ferment mannitol. Most non-pathogenic staphylococci will not ferment mannitol. The Staphylococcus aureus ferments mannitol and turns the medium yellow. The Serratia marcescens does not grow because of the high salt content.

What bacteria can grow on Cetrimide Agar?

Cetrimide agar is a type of agar used for the selective isolation of the gram-negative bacterium, Pseudomonas aeruginosa. As the name suggests, it contains cetrimide, which is the selective agent against alternate microbial flora.

Does Enterobacter aerogenes Grow on mannitol salt agar?

This test showed the bacterium did not contain the enzyme urease. Four more tests, Casein, Starch, Simmons Citrate and a Nitrate test, were all performed to confirm the bacterium was Enterobacter aerogenes. This resulted in the growth of bacteria and the MSA test was concluded to be positive for mannitol fermenters.

What makes mannitol salt agar selective?

Mannitol Salt Agar (MSA) is a selective and differential medium. The high concentration of salt (7.5%) selects for members of the genus Staphylococcus, since they can tolerate high saline levels. Most pathogenic staphylococci, such as Staphylococcus aureus, will ferment mannitol.

Will Bacillus Grow on mannitol salt agar?

Some group D enterococci may exhibit growth with mannitol fermentation; however, catalase test and gram morphology should distinguish between enterococci and staphylococci. Prolonged incubation (≥ 48 hours) may also allow growth of Micrococcus, Bacillus, and some species of Serratia.

Does micrococcus Grow on mannitol salt agar?

Mannitol Salt Agar can be used to culture Micrococcus spp as it is selective for certain Gram- positive microorganisms. However, it will also allow growth of Staphylococcus so further identification work must be conducted to form a strong identification of Micrococcus presence.